Expression of COL3A1 mRNA encoding key fibrotic extracellular matrix molecules was down-regulated by pre-miRNA-29b. The results point to the fact that the injury and/or mandible fracture increase the collagen type III metabolism and its dynamics depends on the type of the used bone fixation COL3A1 (EDS4A) Tissue specificityi. The RNA specificity category is based on mRNA expression levels in the analyzed samples based on a combination of data from HPA, GTEX and FANTOM5. The categories include: tissue enriched, group enriched, tissue enhanced, low tissue specificity and not detected. Read more Tissue proteome. GENERAL INFORMATIONi. General description of the gene and the encoded protein (s) using information from HGNC and Ensembl, as well as predictions made by the Human Protein Atlas project. Gene namei. Official gene symbol, which is typically a short form of the gene name, according to HGNC. COL3A1
On the whole, the expression level of Col3a1 mRNA in muscle of LW was higher than that of LL (P < 0.05). Correlation analysis showed that the expression of Col3a1 mRNA in muscle was positively correlated with total and insoluble IMC, but was negatively correlated with IMC solubility for LW pigs (P < 0.01) and LL pigs (P < 0.05), respectively Type III Collagen is a homotrimer, or a protein composed of three identical peptide chains (), each called an alpha 1 chain of type III collagen.Formally, the monomers are called collagen type III, alpha-1 chain and in humans are encoded by the COL3A1 gene.Type III collagen is one of the fibrillar collagens whose proteins have a long, inflexible, triple-helical domain
Patients were divided based on level of expression into one of the two groups low (under cut off) or high (over cut off). X-axis shows time for survival (years) and y-axis shows the probability of survival, where 1.0 corresponds to 100 percent. Read more. COL3A1 is not prognostic in colorectal cancer Expression scores of expression calls use the minimum and maximum Rank of the species to normalize the expression to a value between 0 and 100. Low score means that the gene is lowly expressed in the condition Expression : Phenotypes: Gene Literature (3) GO Terms (6) Nucleotides (487) Proteins (42) Interactants (235) Wiki: XB-GENEPAGE-5864291. Gene Symbol: col3a1 Gene Name: collagen, type III, alpha 1 (Ehlers-Danlos syndrome type IV, autosomal dominant) Anatomy terms
Kaplan-Meier survival analysis revealed that COL3A1 expression was associated with shorter overall survival in the four major histotypes of epithelial ovarian carcinoma patients (P value = 0.026, HR = 2.99 (95% CI 1.089-8.19)). Furthermore, GPR158 and PITHD1 were shown to be histotype-specific prognostic biomarkers, with elevated GPR158. Gene with a very high level of expression: COL3A1. Expression of collagen (COL) proteins from groups 2 and 3 were also confirmed using immunocytochemistry. Western blot analysis showed very high expression levels of COL3A1 protein, and immunocytochemistry analysis showed the presence of extracellular COL3A1 in the W1TR cell line Overexpression of lnc-GULP1-2:1 in KGN cells promoted the expression of COL3A1 and its translocation into the nucleus. Consistently, silencing COL3A1 in KGN cells also significantly inhibited cell proliferation View mouse Col3a1 Chr1:45350698-45388866 with: phenotypes, sequences, polymorphisms, proteins, references, function, expression The influence of COL3A1 on DMFS of TNBC patients analyzed by KM-plotter is shown in Figure 6B, the DMFS of TNBC patients with high-expression COL3A1 was shorter than in those with a low expression thereof. There was no significant difference in the expression level of COL3A1 and DMFS in the overall patients
Lnc-GULP1-2:1 affects the proliferation of granulosa cells by regulating the expression and localization of COL3A1 protein, and may participate in the regulation of ovarian follicle development. This study will provide new insight into molecular mechanisms underlying ovarian follicular development, COL3A1 overexpression promotes metastasis of triple-negative breast cancer and is linked to a poor prognosis in human bladder cancer. COL5A1 contributes to the metastasis of lung adenocarcinoma and clear cell renal cell carcinoma and can suppress the progression of breast invasive ductal carcinoma Col3a1 collagen type III alpha 1 chain [ (Norway rat)] the activation of Pin1 promoted cardiac extracellular matrix deposition and oxidative stress damage by regulating the phosphorylation of the MEK1/2ERK1/2 signaling pathway and the expression of alphaSMA. the diagnostic value of urinary collagen degradation products in a proteinuria-driven. The expression of fibrosis and scar markers, Col3a1 and αSMA, as well as EGFP expression, was significantly reduced after EV treatments, as compared to wound controls (Fig. 4D). Lastly, we did not observe any gender effect on the treatment outcome
COL3A1 gene expression. In view of this, this study ex-plored the expression level of lnc-GULP1-2:1 in granu-losa cells of patients with different ovarian functions and its influence on granulosa cell proliferation and cell cycle regulation, and further investigated the role of lnc-GULP1-2:1 in the modulation of COL3A1 expression . TGF-β1 expression was evaluated by immunohistochemistry, and histology was conducted to assess the development of liver fibrosis
(A) COL3A1 expression was correlated with the disease progression of BCa (GSE13507). (B) and (C) Oncomine database indicated that COL3A1 was up-regulated in BCa, compared with normal bladder, as. CAT#: HP200076. The primer mix has been tested to generate satisfactory qPCR data on ABI 7900HT by using the following PCR program: Stage 1: Activation: 50 °C for 2 min; Stage 2: pre-soak:95 °C for 10 min; Stage 3: Denaturation: 95 °C for 15 sec, Annealing: 60°C for 1 min; Stage 4: Melting curve: 95°C for 15 sec, 60°C for 15 sec, 95°C. The COL3A1 gene encodes for the chains of type III procollagen. Collagen type III have been found in the lamina cribrosa and in the TM [102, 103]. A study that explored the expression of genes using expression profiles data from TM tissue, found a different expression of COL3A1 gene in patients with POAG compared to controls
Type III collagen is a fibrillar-forming collagen comprising 3 alpha-1 (III) chains and is expressed in early embryos and throughout embryogenesis. In adult, type III collagen is a major component of the extracellular matrix in a variety of internal organs and skin (Liu et al., 1997). Cloning and Expression A Relative expression of COL3A1 in glioma (LGG and GBM) and normal brain tissue. After COL3A1 siRNA transfected, SHG44 and A172 ( B ) cells showed reduced COL3A1 levels measured by qRT-PCR. C Wound healing assays indicated that the migratory ability of glioma cells was reduced after transfection COL3A1 (Collagen Type III Alpha 1 Chain) is a Protein Coding gene. Diseases associated with COL3A1 include Ehlers-Danlos Syndrome, Vascular Type and Polymicrogyria With Or Without Vascular-Type Ehlers-Danlos Syndrome.Among its related pathways are Endothelins and Collagen chain trimerization.Gene Ontology (GO) annotations related to this gene include integrin binding and SMAD binding The COL3A1 gene provides instructions for making type III collagen. Collagens are a family of proteins that strengthen and support many tissues in the body. Type III collagen is found in the skin, lungs, intestinal walls, and the walls of blood vessels. The components of type III collagen, called pro-α1 (III) chains, are produced from the.
The expression of COL1A1 and COL3A1 was upregulated in cardiac fibroblasts transfected with adenovirus containing circHIPK3, while their expression was downregulated when fibroblasts were co-transfected with circHIPK3 and miR-29b-3p mimic (Fig. 7e) Collagen III (COL3A1) (NM_000090) Human Over-expression Lysate. CAT#: LY400026 Reviews Write a review. Transient overexpression lysate of collagen, type III, alpha 1 (COL3A1) Datasheet. SDS View other COL3A1 proteins (3) BOGO250! Buy one, get 50% off the 2nd overexpression lysate. USD 550.00. Availability* 5 Days. Size. 100 ug; Size. Request. Expression of Col1 and Col3 were determined via reverse transcription-quantitative polymerase chain reaction with target genes of Collagen Type I Alpha 1 Chain (COL1A1) and Collagen Type III Alpha 1 Chain (COL3A1) Alterations in collagen type I α1 (COL1A1) and collagen type I α 2 (COL1A2) expressionlevels have been reported to predict prognosis in various types of cancer. However, the effect of these biomarkers on hypopharyngeal squamous cell carcinoma (HPSCC) is yet to be fully elucidated. The present study aimed to explore the prognostic significance of COL1A1 and COL1A2 expression levels in HPSCC
A novel mutation in COL3A1 associates to vascular Ehlers-Danlos syndrome with predominant musculoskeletal involvement. Federica Ruscitti, Lucia Trevisan, Giulia Rosti, Fabio Gotta, Our findings provide insight into genetic variants and clinical expression of vEDS, broadening the clinical scenario of the syndrome.,. Col3a1 expression was prominent in the vascular wall, lung mesenchyme, peritoneal lining and the wall of visceral organs. Col3a1was co-expressed with Col1a1 in skin, but not in bone. None of the sense probes for any of the collagen or ADAMTS genes gave a signal above background levels Finally, we found that COL3A1 mRNA expression was upregulated in skin fibroblasts from patients 1 and 2 with the N-terminal bi-allelic mutation. Although this effect was not visible at the protein level and no differences in protein patterns could be detected after western blot analysis, the involvement of another pathway should be considered
CAT#: MP202801. The primer mix has been tested to generate satisfactory qPCR data on ABI 7900HT by using the following PCR program: Stage 1: Activation: 50 °C for 2 min; Stage 2: pre-soak:95 °C for 10 min; Stage 3: Denaturation: 95 °C for 15 sec, Annealing: 60°C for 1 min; Stage 4: Melting curve: 95°C for 15 sec, 60°C for 15 sec, 95°C. Importantly, COL3A1 expression was inversely correlated with let-7d levels in RCC clinical specimens. Our findings suggest the involvement of the let-7d-COL3A1 regulatory pathway in RCC growth and metastasis. Macrophages, which are abundant in the tumor microenvironment,.
For more info on COL3A1, check out the COL3A1 Infographic We have 30,000+ of these available, one for each gene! check them out. In this infographic you will see the following information for COL3A1: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related. According to the expression profile of uterine cervix, COL3A1 is highly expressed in uterine cervix. So, it is likely that anti-Collagen III/COL3A1 antibody (monoclonal, 9H9) M00788 will work for IHC-P with uterine cervix
COL3A1 (Myc-DDK-tagged)-Human collagen, type III, alpha 1 (COL3A1) Expression varies depending on the nature of the gene. Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers COL3A1 expression was associated with ECM receptor interaction, regulation of actin cytoskeleton and focal adhesion pathways via GSEA analysis. In conclusion, COL3A1 may be a molecular biomarker for prognosis and immune infiltration in pan-cancer. It might act as a potential target for a new insight of human cancers management COL3A1 HEK293T cell transient overexpression lysate (as WB positive control COL3A1 expression changes between the normal tissues and different cancer tissues of CRC by TCGA dataset. The n indicates the total cases of different categories. The p values are shown above the transverse lines, which are calculated using two-tailed and unpaired Student's t test Expression System: E.coli: Purity > 90% pure: Molecular Weight: Form & Buffer: Supplied in liquid form in 6M guanidine hydrochloride and 20mM Tris buffer See all options for COL3A1 protein. Storage & Safety. Storage: Store at 4 deg C in a working aliquot for 1 week. For long term storage, aliquot and freeze at -20 to -80 deg C, avoid repeat.
COL3A1 Transcriptome. HumanBase was utilized to generate glomerular and tubular COL3A1 transcriptomes i.e., the network of genes/proteins modulated by a change in the COL3A1 expression level from baseline/healthy kidneys. The glomerular (A) COL3A1 transcriptome (average strength of 0.8 ± 0.08, degree o The relative of COL3A1 expression levels on mRNA: (a) pPZ-2 (COL3A1-2); (b) pPZ-3 (COL3A1-3); (c) pGAP-4 (COL3A1-4); (d) pGAP-5 (COL3A1-5). In conclusion, to obtain hydroxylated rhCOL3A1, pGAPZ α B-P4H should be chosen as the fermentation strain on account of high and stable expression of recombinant P4H gene at mRNA level, and has a. MiR-29b, COL1A1, COL2A1, COL3A1 and SOX9 gene expression of BMSC cocultured with OA cartilage and OA bone explants. (a) miR-29b (white bars), COL1A1 (light grey bars), COL3A1 (dark grey bars) and.
Gene expression in human CRC microarray dataset. Type I alpha I collagen (COL1A1), type III alpha I collagen (COL3A1), ELN, MMP9, MMP12, TIMP3, TNF gene expressions were from existing microarray datasets through Gene Expression Omnibus (GEO).The data were analyzed using Bioconductor in R as previously described [13, 14].In GSE128449 dataset, gene microarray from colorectal tissues were. The expression vector-mediated RNAi established a HSF cell line with persistent gene knockdown for over 30 days (to 25.21% and 22.12%, respectively). CONCLUSION: COL1A1 and COL3A1 gene expressions can be specifically and efficiently inhibited in HSFs by either liposome- or vector-mediated SEC transfection Collagen type III occurs in most soft connective tissues along with type I collagen. Involved in regulation of cortical development. Is the major ligand of ADGRG1 in the developing brain and binding to ADGRG1 inhibits neuronal migration and activates the RhoA pathway by coupling ADGRG1 to GNA13 and possibly GNA12 COL3A1 (B-10): sc-271249.Western blot analysisof COL3A1 expression in HISM (A)andCCD-1064Sk (B) wholecell lysates and human bone marrow tissue extract (C). 114K- AB C COL3A1 COL3A1 (B-10) HRP: sc-271249 HRP. Direct western blot analysis of COL3A1 expression in non-transfected 293T: sc-117752 (A), human COL3A1 transfected 293T
Col3a1. 245. Annotation score: Annotation score:1 out of 5. The annotation score provides a heuristic measure of the annotation content of a UniProtKB entry or proteome. This score cannot be used as a measure of the accuracy of the annotation as we cannot define the 'correct annotation' for any given protein Gene expression Figures 2 and 3 show the relative expression distribution of COL1A1, COL3A1, DEFB4B, FOXP3, IDO1, IL6, MMP9, TGFB1, TGFBR1 and TNFA transcripts of uter-ine biopsy of 25 horses grouped by age (younger and older than 10years). The expression values are normal-ized with 3 reference genes: glyceraldehyde-3-phosphat A, Expression of ACTA2 in HAFs by immunofluorescent staining (scale bar is 50 μm). B, Col1a1, Col3a1 and ACTA2 expression was detected using western blot assay in Ang-II-induced HAFs. Expression of miR-23b/27b precursor (C), miR-23b-3p and miR-27b-3p (D) was determined using RT-qPCR assay in Ang-II-induced HAFs. Data are shown as mean ± SEM. animals Communication Dietary Lecithin Decreases Skeletal Muscle COL1A1 and COL3A1 Gene Expression in Finisher Gilts Henny Akit 1,2,*, Cherie Collins 3, Fahri Fahri 1, Alex Hung 1, Daryl D'Souza 4, Brian Leury 1 and Frank Dunshea 1 1 Faculty of Veterinary and Agricultural Sciences, The University of Melbourne, Parkville, Victoria 3010, Australia; Fahri.Fahri@foodauthority.nsw.gov.au (F.F.
The expression of α‐SMA, COL1A1, COL3A1, TIMP‐1 was determined by immunohistochemistry. The RT‐qPCR, Western blotting and immunofluorescence staining were conducted to determine expression of miR‐29b‐3p, COL1A1, and COL3A1. CCK‐8 assay and flow cytometry were performed to evaluate viability and apoptosis were performed to evaluate mRNA and protein expression of miR-133b targets, E-cadherin, α-smooth muscle actin (SMA), fibronectin, and collagen 3A1 (Col3A1), in HK2 cells transfected with miR-133b under TGF-β1 stimulation. A 24-month-old unilateral ureteral obstruction (UUO) mouse model was established and injected with transfectio
The expression of Col1a1 was significantly reduced in the LM+GM group on days 7 and 15 postinjury (Fig. 3C). In contrast, the Col3a1 gene expression profile was similar between the LM and LM+GM groups, and the expression in both groups peaked on day 7 and returned to baseline on day 30 enhanced expression of COL3A1 and CCL7 genesin vitro. The mRNA levels of COL3A1 and CCL7 were inversely correlated with let-7d level in RCC clinical specimens. Conclusions:These results suggest that let-7d may suppress RCC growth, metastasis, and tumor macrophage infiltration at least partially through targeting COL3A1 and CCL7 Western blot analysis revealed that CvME significantly upregulated the expression of COL3A1 and bFGF and increased the Smad-mediated collagen production in granulation tissue. These findings suggest that C. viscosa promoted the wound repair process by attenuating the Smad-mediated collagen production in wound granulation tissue Expression of one of these proteins, C1r (complement component 1), is shown to be entrained to the vascular smooth muscle PTH1R-Mkl-1 signal relay controlling Col3a1 and Col1a1 expression. Discovery of this connection may lead to novel approaches to monitor and inhibit arterial fibrosis
We found positive correlations between COL3A1 and COL5A1 expression in GC cells, and confirmed that miR-29a-3p is the common regulator of their expression. RNA immunoprecipitation assays based on Ago2, Dicer, and exportin-5 showed that SFA could promote mature miR-29a-3p generation with expression of its targets, COL3A1 and DNMT3A. Gain of function of miR-29c inhibited the expression of COL3A1 and DNMT3A at protein and mRNA levels, secreted COL3A1, and rate of cell proliferation. Loss of function of miR-29c had the opposite effect. E 2, P, and their combination inhibited miR-29c in leiomyoma smooth muscle cells (LSMC. Collagen, type III, alpha-1 (COL3A1) is essential for normal collagen I fibrillogenesis in many organs. There are differences in phenotypes of mutations in the COL3A1 gene in humans and mutations in mice. In order to investigate whether the regulation and gene network of COL3A1 is the same in healthy populations of mice and humans, we compared the quantitative trait loci (QTL) that regulate. Collagen type III occurs in most soft connective tissues along with type I collagen. Involved in regulation of cortical development. Is the major ligand of ADGRG1 in the developing brain and binding to ADGRG1 inhibits neuronal migration and activates the RhoA pathway by coupling ADGRG1 to GNA13 and possibly GNA12. This gene encodes the pro-alpha1 chains of type III collagen, a fibrillar.